0
ARTICLE |

DEMONSTRATIONS OF EXPERIMENTAL WORK IN THE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE:  11. CYTOLOGY OF SEROUS EFFUSIONS, WITH SPECIAL REFERENCE TO TUMOR CELLS

ALFRED GOLDMAN, M.D.
Arch Surg. 1929;19(6):1672-1678. doi:10.1001/archsurg.1929.01150060734042.
Text Size: A A A
Published online

The study of the cellular structure of effusions is a valuable laboratory procedure. The most satisfactory method of study is that devised by Mandlebaum.1 The fluid is placed in a large Erlenmeyer flask and allowed to stand over night in the icebox. The supernatant fluid is decanted and the sediment poured in a large 50 cc. centrifuge tube and centrifugated for at least twenty minutes at a moderate speed. The supernatant fluid is again decanted and the sediment hardened with a diluted solution of formaldehyde, U. S. P. (1: 10) or Zenker's fluid for twenty-four hours. The fixed sediment is then treated as ordinary tissue by running through alcohols, embedding in paraffin and staining with hematoxylin eosin. The tissue is cut from above down so as to include all the cellular elements.

This report is based on the study of fifty effusions prepared by this method from serous fluids

Topics

Sign In to Access Full Content

Don't have Access?

Register and get free email Table of Contents alerts, saved searches, PowerPoint downloads, CME quizzes, and more

Subscribe for full-text access to content from 1998 forward and a host of useful features

Activate your current subscription (AMA members and current subscribers)

Purchase Online Access to this article for 24 hours

First Page Preview

View Large
/>
First page PDF preview

Figures

Tables

References

Correspondence

CME
Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).
Submit a Comment

Multimedia

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

Related Content

Customize your page view by dragging & repositioning the boxes below.

Jobs
brightcove.createExperiences();