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HISTOLOGY OF HEALING FRACTURES IN RATS ON DIETS LOW IN TOTAL SALT, CALCIUM AND PHOSPHORUS

WILLIAM G. DOWNS Jr., D.D.S., Ph.D.; RAYMOND M. McKEOWN, M.D
Arch Surg. 1932;25(1):108-121. doi:10.1001/archsurg.1932.01160190111006.
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Previous reports by Lindsay and Howes,1 McKeown, Lindsay, Harvey and Lumsden,2 and Downs and McKeown3 have dealt with the breaking strength and histology of fractured fibulae in rats on normal and synthetic diets. The present study deals similarly with variations observed microscopically in the fractured fibulae of these animals when the salt content of their diet was markedly altered.

METHOD  As in the previous studies, the animals were placed on the diet utilized for one week, at the expiration of which time the right fibula was fractured in the manner described before.1 Two animals from each dietary group were killed at the following postoperative time periods: six, twelve, eighteen and twenty-four hours. Two animals were also killed one, two, three, four, five and six days postoperatively and thereafter at three day intervals to and including fifty-one days. After death the legs were removed, and the tibiae

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