This is a description of an experimental method for producing hyperamylasemia in which the animal's pancreas itself is not involved. The elevations of serum and lymphatic amylase produced in this way will be compared with those produced by injection of pancreatic juice intravenously, intraperitoneally, and retroperitoneally.
Pancreatic juice was obtained from six dogs by cannulation of the large pancreatic duct with a small polyethylene tube, after ligature and division of the small pancreatic duct. After recovery from anesthesia, the dogs stood or lay quietly during collection of the pancreatic juice and the measurement of pancreatic secretory pressures. The flow of pancreatic juice was stimulated by small feedings of horse meat. The pancreatic juice was collected in iced flasks and was stored at 4 C. No other attempts were made to minimize bacterial growth. The organisms cultured from the pancreatic juice included Pseudomonas aeruginosa, α-hemolytic streptococcoci, and coagulase-negative staphylococci. Pressure