At present, the only long-term storage method for tissues and organs is freezing with glycerol. Much work has been done in establishing low-temperature storage norms for tissues such as spermatozoa,6,7 blood,3,8 bone,5 arteries,4 skin,2 and endocrine organs.9 Little or no successful long-term cold storage work has been done with the mammalian heart, though freezing down to almost absolute zero has been accomplished with transient recovery.1 In the studies and results obtained and herein reported, rats' hearts were successfully stored in glycerol at temperatures ranging from 25 C to 0 C.
Rats were anesthetized with pentobarbital, injected with 0.25 mg. of heparin,10 and exsanguinated by severing the abdominal aorta. The rib cage was opened, and the intact heart, lungs, and aorta were excised. A cannula made from a blunted 18-gauge needle was tied into the aorta with its tip just above the