0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
ARTICLE |

Preservation of Hearts by Freezing

ARMAND M. KAROW JR., BA; WATTS R. WEBB, MD; JOHN E. STAPP
Arch Surg. 1965;91(4):572-574. doi:10.1001/archsurg.1965.01320160026005.
Text Size: A A A
Published online

PRESERVATION of mammalian organs by hypothermia requires methods of cooling and warming that will maintain uniform temperature distribution throughout the tissues, yet permit changes in temperature at a sufficiently rapid rate to equalize physical stress and minimize variations in biochemical reaction rates.3 The freezing of cells has been most successful when performed relatively slowly (in the range of 1 to 10 C per minute) in the temperature range from 0 to 20 C with rapid freezing thereafter. This present work has sought the limits of freezing of organs with best available techniques and protective agents.

Methods  Albino rats averaging 200 gm received 25 mg pentobarbital (Nembutal) and 5 mg sodium heparin intraperitoneally. The aorta was severed and an aortic cannula was lodged just above the coronary arteries. The heart and lungs were removed in toto and placed on a modified Langendorff perfusion column (Fig 1). After perfusion commenced the

Topics

Sign in

Create a free personal account to sign up for alerts, share articles, and more.

Purchase Options

• Buy this article
• Subscribe to the journal

First Page Preview

View Large
First page PDF preview

First Page Preview

View Large
First page PDF preview

Figures

Tables

References

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Submit a Comment

Multimedia

Some tools below are only available to our subscribers or users with an online account.

Sign in

Create a free personal account to sign up for alerts, share articles, and more.

Purchase Options

• Buy this article
• Subscribe to the journal

Related Content

Customize your page view by dragging & repositioning the boxes below.

Jobs
brightcove.createExperiences();