WITH THE increasing clinical use of hyperbaric oxygenation, much has been said about the effect of high oxygen tensions on anaerobic organisms, but very little information is available concerning the effect of high oxygen tensions on aerobic infections, particularly in vivo. It is the purpose of these experiments to demonstrate the effects of hyperbaric oxygenation on four well-controlled and well-established in vivo systems of aerobic bacterial infections.
Methods and Results
In this series of experiments, male, adult Sprague-Dawley rats were utilized. Anesthesia for flap excision or burning always consisted of pentobarbital, 1 mg/25 gm. Treatment with oxygen at normal pressures and also compression with oxygen or air to 3 atmospheres absolute (3 ATA) was carried out in an experimental chamber with an adequate flow-through system to prevent CO2 buildup. Five sets of experiments were carried out.
—A dorsal full-thickness suprafascial flap was created over 20% of the