THE PERPLEXING dilemma of whole-organ procurement and storage is not the least of the problems associated with transplantation procedures.
Previously we reported successful preservation in vitro of canine whole organs by combined hypothermia and hyperbaric oxygen for periods up to 48 hours.1,2,3 The temperature for these studies was maintained fairly constant between 2 to 4 C, while the hyperoxia varied from 3.0 to 8.0 atmospheres, the higher pressure generally being associated with extension of storage from 24 to 48 hours. A notable exception was the invariable finding that the lung would less tolerate in vitro exposure to hyperbaric oxygen above 3.0 atm for any period.4 This observation formed the framework for further investigation.
Materials and Methods
Method of Organ Preservation
—A commercially available organ-preservation unit manufactured to our specifications (providing both hypothermia and hyperbaric oxygenation) was used. This unit has three hyperbaric chambers, each with a volume capacity