Liver preservation was attempted with 39 canine livers. Of 36 orthotopically transplanted livers, there were 17 failures because of anesthetic overdosage and respiratory problems. Nineteen dogs receiving livers preserved for 24 hours were eligible for evaluation of success of preservation. Twelve of the 19 dogs survived 5 to 70 days after transplantation, indicating successful preservation in most instances. Factors that may have contributed to success were administration of large doses of methylprednisolone to the donor prior to liver harvest, a relatively simple and efficient transplantation technique, low temperature of perfusion, and inclusion of high-dose methylprednisolone in the perfusate.