We're unable to sign you in at this time. Please try again in a few minutes.
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Article |

Role of the Macrophage in the Translocation of Intestinal Bacteria

Carol L. Wells, PhD; Michael A. Maddaus, MD; Richard L. Simmons, MD
Arch Surg. 1987;122(1):48-53. doi:10.1001/archsurg.1987.01400130054008.
Text Size: A A A
Published online


• To clarify the role of the macrophage in the translocation of intestinal bacteria, groups (n = 10) of Swiss Webster mice (immunocompetent) and C3H/HeJ mice (macrophage defective) were given bacitracin/streptomycin in their drinking water to eliminate the majority of the intestinal microflora. These mice were then "monoassociated" with a streptomycin-resistant strain of Escherichia coli. Forty-eight hours later, E coli was present in all animals at a concentration of 1011/g of cecum. In four separate experiments, E coli was recovered from 100% of the mesenteric lymph nodes (MLNs) of the immunocompetent Swiss Webster mice and from 10%, 40%, 30%, and 50% of the MLNs of macrophage-defective C3H/HeJ mice. Swiss Webster mice were then similarly monoassociated by antibiotic decontamination followed by administration of antibiotic-resistant, fluorescein-labeled E coli in their drinking water; cohort groups of mice were given fluorescein-labeled latex beads (1 μm in diameter) in their drinking water. Two, four, and 11 days later, the MLNs were removed and single cell suspensions were analyzed in the fluorescence-activated cell sorter. The fluorescein label was detected exclusively in the macrophage (esterase-positive) population. These results support the hypothesis that intestinal macrophages may play a key role in the transport of intestinal particles (including bacteria) into extra-intestinal sites.

(Arch Surg 1987;122:48-53)


Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?





Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.


Some tools below are only available to our subscribers or users with an online account.

0 Citations

Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?

Related Content

Customize your page view by dragging & repositioning the boxes below.