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ARTICLE |

Induction of Heat Shock Protein 72 Prevents Neutrophil-Mediated Human Endothelial Cell Necrosis

Jiang Huai Wang, MD, PhD; H. Paul Redmond, MCh, FRCSI; R. William G. Watson, PhD; Claire Condron, BSc; David Bouchier-Hayes, MCh, FRCSI
Arch Surg. 1995;130(12):1260-1265. doi:10.1001/archsurg.1995.01430120014002.
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Objective:  To examine the hypothesis that induction of heat shock proteins in human endothelial cells (ECs) by either heat shock or sodium arsenite could prevent subsequent EC necrosis induced by activated human polymorphonuclear neutrophil leukocytes (PMNs).

Design:  Cultures of ECs were exposed to heat shock (42°C, 30 to 60 minutes) or sodium arsenite (40 to 320 μmol/L) for 6 hours to induce the expression of a heat shock protein of 72-kd molecular weight (HSP-72). Activated PMNs were subsequently added to these ECs for 24 hours to evaluate the ability of HSP-72 to prevent activated PMN-mediated EC necrosis.

Results:  Neither EC necrosis nor apoptosis was induced by heat shock. Sodium arsenite (40 to 80 μmol/L) did not induce EC necrosis, although 320-μmol/L sodium arsenite caused a significant increase in EC necrosis. Sodium arsenite (80 to 320 μmol/L) also induced dose-dependent EC apoptosis. Endothelial cells exposed to heat shock and sodium arsenite (40 and 80 μmol/L) significantly attenuated subsequent EC necrosis induced by activated PMNs. However, sodium arsenite at 320 μmol/L aggravated activated PMN-mediated EC necrosis. Expression of HSP-72 was detected after ECs were treated both with heat shock and sodium arsenite (40 to 320 μmol/L) for 6 hours.

Conclusion:  Induction of HSP-72 in ECs by a thermal or nonthermal mechanism could prevent activated PMN-mediated EC necrosis, which may favor increased vascular permeability during systemic inflammatory response syndrome.(Arch Surg. 1995;130:1260-1265)

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