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Article |

Effect of Hyperbaric Oxygen on Neutrophil Concentration and Pulmonary Sequestration in Reperfusion Injury

William A. Zamboni, MD; Howard P. Wong, MD; Linda L. Stephenson, MT
Arch Surg. 1996;131(7):756-760. doi:10.1001/archsurg.1996.01430190078020.
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Objective:  To evaluate the effect of hyperbaric oxygen (HBO) on muscle flap arterial neutrophil concentration and pulmonary neutrophil sequestration following ischemia-reperfusion injury in a rat gracilis muscle microcirculation model.

Design:  Prospective randomized experimental trials.

Setting:  Laboratory.

Materials:  Male Wistar rats weighing 150 to 300 g.

Interventions:  Gracilis muscle flaps were raised on isolated vascular pedicles. Three groups were evaluated: (1) sham (flap raised, 4-hour observation, no ischemia, no HBO), (2) ischemia control (4-hour flap ischemia, no HBO); and (3) ischemia (4-hour flap ischemia) plus HBO (last 90 minutes of ischemia, 100% oxygen, 2.5 atm absolute).

Main Outcome Measures:  Flap pedicle arterial leukocyte and neutrophil concentrations were assessed at 5 and 90 minutes of reperfusion in the 3 groups. Pulmonary neutrophil sequestration was measured in a blinded fashion, using histologic examination and myeloperoxidase assay in the 3 groups at 5 and 90 minutes of reperfusions.

Results:  Ischemia produced an increase in pedicle arterial leukocyte and neutrophil concentrations in blood flowing to the gracilis muscle flap; administration of HBO significantly reduced concentration to sham levels (P<.05). There were no significant differences in pulmonary neutrophil sequestration among the experimental groups.

Conclusions:  In this rat gracilis muscle microcirculation model, the increase in pedicle arterial leukocyte and neutrophil concentrations following ischemia-reperfusion injury was significantly reduced to sham levels by HBO treatment. This observed reduction was not attributable to HBO-induced pulmonary sequestration, which did not significantly change with HBO administration. Further investigation is required to elucidate the mechanisms of action of HBO in ameliorating ischemia-reperfusion injury in this model.Arch Surg. 1996;131:756-760


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