December 1996, Vol 131, No. 12 >

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ARTICLE | December 1996

Exudative Neutrophils Show Impaired pH Regulation Compared With Circulating Neutrophils

David J. Hackam, MD; Sergio Grinstein, PhD; Avery Nathens, MD; R. William G. Watson, PhD; John C. Marshall, MD; Ori D. Rotstein, MD

Arch Surg. 1996;131(12):1296-1301. doi:10.1001/archsurg.1996.01430240050006.

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Objective: To investigate the effects of neutrophil (PMN) transmigration into inflammatory sites on cytosolic pH (pH_i) regulation.

Design: Exudative PMNs were obtained by bronchoalveolar lavage from rats sustaining endotoxin-induced lung injury. Circulating PMNs were purified with density-gradient centrifugation. Cytosolic pH was measured with single-cell fluorescence imaging using the pH-sensitive dye biscarboxyethyl-carboxyfluorescein.

Results: Exudative PMNs showed impaired pH, recovery from an induced acid load compared with circulating PMNs. Under conditions of extracellular acidosis, exudative PMNs showed impaired pH_i homeostasis and produced decreased superoxide compared with circulating cells. Inhibition of the sodiumproton exchanger attenuated the differences in pH_i recovery, suggesting a mechanism underlying the pH_i regulatory dysfunction. All cells had comparable adenosine triphosphate levels and superoxide production at physiologic extracellular pH.

Conclusion: Impaired pH, regulation of exudative cells may mediate cellular dysfunction and impaired resolution of infection at inflammatory sites.Arch Surg. 1996;131:1296-1301

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Hackam DJ, Grinstein S, Nathens A, Watson RG, Marshall JC, Rotstein OD. Exudative Neutrophils Show Impaired pH Regulation Compared With Circulating Neutrophils. Arch Surg. 1996;131(12):1296-1301. doi:10.1001/archsurg.1996.01430240050006.

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