TY  - JOUR
T1  - L-selectin and leukocyte function in skeletal muscle reperfusion injury
AU  - Lozano DD, Kahl EA, Wong HP, Stephenson LL, Zamboni WA
Y1  - 1999/10/01
N1  - 10.1001/archsurg.134.10.1079
JO  - Archives of Surgery
SP  - 1079
EP  - 1081
VL  - 134
IS  - 10
N2  - Hypothesis 
    Treatment with anti–L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle.Design 
    Prospective, randomized experimental trials.Setting 
    Basic science research laboratory.Materials 
    Male Wistar rats weighing 109±5 g (mean±SEM).Interventions 
    Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy. Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti–L-selectin monoclonal antibody 30 minutes before reperfusion.Main Outcome Measures 
    The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion.Results 
    Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P<.05). Use of the monoclonal antibody significantly reduced the number of adherent neutrophils at 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion (P<.05). There was no significant difference in leukocyte velocity.Conclusion 
    L-Selectin plays a significant role in leukocyte rolling and adherence to venular endothelium in rat skeletal muscle ischemia reperfusion injury.
SN  - 0004-0010
M3  - doi: 10.1001/archsurg.134.10.1079
UR  - http://dx.doi.org/10.1001/archsurg.134.10.1079
ER  -