TY  - JOUR
T1  - PReferential loss of cxcr-2 receptor expression and function in patients who have undergone trauma
AU  - Quaid GA, Cave C, Robinson C, Williams MA, Solomkin JS
Y1  - 1999/12/01
N1  - 10.1001/archsurg.134.12.1367
JO  - Archives of Surgery
SP  - 1367
EP  - 1372
VL  - 134
IS  - 12
N2  - Background 
    In response to traumatic injury or infection, human neutrophils are directed to the site of injury or infection by CXC chemokines that signal via 2 receptors, CXCR-1 and CXCR-2. In vitro studies have shown preferential loss of CXCR-2 expression and function after exposure to interleukin 8, N-formyl-methionyl-leucyl-phenylalanine (fMLP), C5a, and tumor necrosis factor α.Hypothesis 
    CXCR-2 expression and function are preferentially down-regulated in severely injured patients.Methods 
    We studied 20 patients within 24 hours of admission to the hospital. Patients with head injuries were excluded. Injury Severity Scores (range, 1-50; mean, 35) were calculated for each patient. To determine expression of CXCR-1 and CXCR-2, flow cytometry was used. Intracellular calcium mobilization and neutrophil migration to 10 nmol of interleukin 8, growth-related oncogene α, and fMLP was measured to determine receptor function.Results 
    Compared with CXCR-1, there is a greater loss of CXCR-2 receptor expression in the severely injured group (P = .01). Neutrophils from patients with Injury Severity Scores greater than 16 did not mobilize calcium in response to growth-related oncogene α. However, there was no loss of calcium mobilization to interleukin 8 or fMLP. Chemotaxis to various stimulants is decreased in all injury groups.Conclusions 
    CXCR-2 expression and function are preferentially down-regulated in severely injured patients. Our data suggest that there are multiple mechanisms, in addition to receptor down-regulation, that play a role in the loss of migration and calcium flux in human neutrophils after injury.
SN  - 0004-0010
M3  - doi: 10.1001/archsurg.134.12.1367
UR  - http://dx.doi.org/10.1001/archsurg.134.12.1367
ER  -