TY  - JOUR
T1  - EFfect of diabetes mellitus on endotoxin-induced lung injury
AU  - Wright J, Nwariaku FN, Clark J, Falck J, Rogers T, Turnage RH
Y1  - 1999/12/01
N1  - 10.1001/archsurg.134.12.1354
JO  - Archives of Surgery
SP  - 1354
EP  - 1359
VL  - 134
IS  - 12
N2  - Objective 
    To examine the effects of diabetes mellitus on lipopolysaccharide (LPS)–induced pulmonary edema and alveolar neutrophil recruitment and activation.Hypothesis 
    Zucker diabetic fatty rats are resistant to the effects of intratracheal LPS on the extravasation of plasma proteins into the lungs.Design 
    Zucker diabetic fatty (ZDF) rats (genotype fa/fa) were used as a model of diabetes mellitus, while their normoglycemic heterozygous littermates served as controls. Lipopolysaccharide (Escherichia coli 0111:B4; 100-200 µg) or vehicle (0.25 mL of isotonic sodium chloride solution) was instilled into the airways of ZDF and control rats. Four hours later, pulmonary microvascular dysfunction was assessed by measuring the extravasation of Evans blue dye into the lung. Lipopolysaccharide-induced neutrophil recruitment was assessed by counting the number of neutrophils within the bronchoalveolar lavage fluid and measuring their expression of CD11b/CD18 by fluorescence-activated cell analysis sorting.Results 
    The LPS (200 µg) induced a 32% increase in Evans blue dye extravasation into the lungs of controls (P = .008) but had no such effect in diabetic animals. Pulmonary extravasation of Evans blue dye in controls was greater than that of ZDF rats both at baseline (P = .002) and in response to 200 µg of LPS (P<.001). The LPS up-regulated neutrophil CD11b/CD18 expression in diabetic and nondiabetic groups and induced a greater than 50-fold increase in the number of neutrophils within the airways of both control and diabetic groups (P<.001).Conclusion 
    Despite the recruitment of a large number of neutrophils into the lung, the LPS-induced change in pulmonary microvascular permeability in diabetic animals is substantially less than that of nondiabetic controls.
SN  - 0004-0010
M3  - doi: 10.1001/archsurg.134.12.1354
UR  - http://dx.doi.org/10.1001/archsurg.134.12.1354
ER  -