TY  - JOUR
T1  - IMproving glucose metabolism with resveratrol in a swine model of metabolic syndrome through alteration of signaling pathways in the liver and skeletal muscle
AU  - Burgess TA, Robich MP, Chu LM, Bianchi C, Sellke FW
Y1  - 2011/05/01
N1  - 10.1001/archsurg.2011.100
JO  - Archives of Surgery
SP  - 556
EP  - 564
VL  - 146
IS  - 5
N2  - Hypothesis 
    We hypothesized that supplemental resveratrol would affect glucose metabolism in the skeletal muscle and liver to improve blood glucose control.Design 
    Case-control study.Setting 
    Hospital laboratory.Subjects 
    Yorkshire miniswine.Intervention 
    The swine developed metabolic syndrome by consuming a high-calorie, high–fat/cholesterol diet for 11 weeks. Pigs were fed either a normal diet (control) (n = 7), a hypercholesterolemic diet (HCC) (n = 7), or a hypercholesterolemic diet with supplemental resveratrol (100 mg/kg/d) (HCRV) (n = 7). Animals underwent dextrose challenge prior to euthanasia and tissue collection.Main Outcome Measures 
    Measurements of glucose and insulin levels, skeletal muscle and liver protein expression, and liver function test results.Results 
    The HCC group had significantly increased blood glucose levels at 30 minutes as compared with the control and HCRV groups. The HCC group demonstrated increased fasting serum insulin levels and levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. Oil red O staining demonstrated increased lipid deposition in the livers of the HCC animals. Immunoblotting in the liver showed increased levels of mammalian target of rapamycin, insulin receptor substrate 1, and phosphorylated AKT in the HCRV group. Immunoblotting in skeletal muscle tissue demonstrated increased glucose transporter type 4 (Glut 4), peroxisome proliferating activation receptor γ coactivator 1α, peroxisome proliferator-activated receptor α, peroxisome proliferator-activated receptor γ, and phosphorylated AKT at threonine 308 expression as well as decreased retinol binding protein 4 in the HCRV group. Immunofluorescence staining for Glut 4 in the skeletal muscle demonstrated increased Glut 4 staining in the HCRV group compared with the HCC or control groups.Conclusion 
    Supplemental resveratrol positively influences glucose metabolism pathways in the liver and skeletal muscle and leads to improved glucose control in a swine model of metabolic syndrome.
SN  - 0004-0010
M3  - doi: 10.1001/archsurg.2011.100
UR  - http://dx.doi.org/10.1001/archsurg.2011.100
ER  -