TY  - JOUR
T1  - THe effect of endotoxin on glucose metabolism in skeletal muscle requires the presence of plasma
AU  - Amaral JF, Shearer JD, Mastrofrancesco B, Gann DS, Caldwell MD
Y1  - 1989/06/01
N1  - 10.1001/archsurg.1989.01410060099021
JO  - Archives of Surgery
SP  - 727
EP  - 732
VL  - 124
IS  - 6
N2  - • The administration of endotoxin in vivo results in an increase in glucose utilization through an as yet undetermined mechanism. This study evaluated (1) the contribution of blood to the increased glucose utilization noted following endotoxemia, (2) the direct action of endotoxin on skeletal muscle glucose uptake in an isolated hindlimb perfusion system and in incubated muscle, and (3) the possibility that the increased glucose uptake in skeletal muscle mediated by endotoxin requires the presence of plasma. Incubation of blood with 50 and 100 mg/L of endotoxin increased glucose uptake and lactate production in a dose-dependent manner. Muscle incubations and perfusions in the absence of plasma and white blood cells showed that glucose uptake and lactate production were not affected by the presence of 50 to 250 mg/L of endotoxin, while 500 mg/L of endotoxin produced a 26.2% decrease in glucose uptake. In contrast, incubation of muscle in the presence of plasma and endotoxin increased glucose uptake by 37%. These findings suggest that (1) the increased glucose utilization of endotoxemia is only partially explained by increased glucose metabolism by blood, (2) endotoxin does not have a direct effect on the glucose uptake of skeletal muscle, and (3) an interaction of endotoxin with a component of plasma is required for an endotoxin-mediated increase in glucose utilization by skeletal muscle.(Arch Surg 1989;124:727-732)
SN  - 0004-0010
M3  - doi: 10.1001/archsurg.1989.01410060099021
UR  - http://dx.doi.org/10.1001/archsurg.1989.01410060099021
ER  -